Chromatography is a laboratory process used to separate the desired components of a mixture or solution. Chromatography vials, often referred to as GC vials, HPLC vials, and ion chromatography vials, are essential in this process. The selection of these vials depends on the type of process, the samples to be analyzed, and other factors. This blog aims to provide a better understanding of chromatography vials.

What are Chromatography Vials?

Chromatography vials are containers used to hold samples for GC (Gas Chromatography) or LC (Liquid Chromatography) analysis. You can easily obtain these vials from a chromatography lab vial supplier.

What Material are Chromatography Vials Made Of?

Chromatography vials are typically made from either plastic or clear glass. Amber-tinted vials are used to protect samples from light exposure. Glass is often chosen for its inertness, while polymers may be used to eliminate interferences during ion chromatography.

The most commonly used plastics are polypropylene (PP) and polymethylpentene (PMP). Both are transparent; PP can withstand heat up to 135 degrees Celsius, while PMP can resist heat up to 175 degrees Celsius.

What is the Standard Size of Chromatography Vials?

Standard sizes for chromatography vials include 8x40mm, 15x45mm, and 12x32mm. A 9mm non-standard vial with a magnetic cap is often used in robotic arm machines. Inserts can be used to reduce the vial's capacity while maintaining its external dimensions, which is useful for smaller samples. These inserts may be conical, flat-bottomed, or pre-fused within the vial. Ensure that the insert matches the vial dimensions.

Features of Chromatography Vials

Some common features of chromatography vials include:

Frosted writing areas

Round or flat bottoms

Graduations

Normal or wide-mouth openings

Thick walls to withstand vacuum or pressure

Even though closures and vials can sometimes be reused, it is not recommended due to the increased risk of contamination from atmospheric or solvent interactions, which could affect the results and necessitate rework.

How Do You Choose Septa?

Septa and liners in chromatography vials are vital for sample preparation and storage. When selecting septa, ensure it is compatible with the solvent. Most septa are faced with PTFE, and when the polytetrafluoroethylene (PTFE) layer is punctured, the solvent may come into contact with the underlying material.

How Do You Choose the Correct Chromatography Vial?

Choosing the correct chromatography vial involves several factors, including:

Choosing the appropriate closure style

Deciding on the vial's volume

Selecting the suitable material (glass, plastic, etc.) based on the requirement

Determining whether an amber or clear vial is needed

Wrapping Up

We hope this blog has provided you with a better understanding of chromatography vials used in laboratories. If you or someone you know is looking to purchase these vials, consider contacting a chromatography vials supplier in the USA to source high-quality vials.